000			02939nam a2200277 a 4500
001			70685
003			BD-RjUL
005			20211209082029.0
008			170419s2006 ii a b 001 0 eng c
020			_a8173715017
035			_a(BD-RjUL)70899
040			_aBTCTA _cBTCTA _dYDXCP _dBAKER _dAGL _dMYG _dOrLoB-B _dBD-RjUL _beng
041			_aeng
082			_223 _a660.6 _bCHM 2006
100	1		_aChannarayappa. _9209211
245	1	0	_aMolecular biotechnology : _bprinciples and practices / _cChannarayappa.
260			_aHyderguda, Hyderabad : _bUniversities Press, _cc2006.
300			_a1217 p. : _bill. ; _c24 cm.
490	1		_aBiotechnology (Universities Press)
504			_aIncludes bibliographical references and index.
505	0	0	_tIntroduction to biotechnology -- _tBiotechnology : scope and importance -- _tBiosafety and good laboratory practices -- _tAdvanced techniques in molecular biology -- _tTechniques of cell fractionation and centrifugation -- _tChemical synthesis of nucleic acids -- _tDNA chip technology and its potential applications -- _tBioinformatics in biotechnology -- _tWorking with nucleic acids -- _tIsolation of nucleic acids -- _tMeasuring nucleic acid concentration and purity -- _tElectrophoretic techniques -- _tDNA sequencing -- _tGenetic maps and marker analysis -- _tPolymerase chain reaction (PCR) -- _tIn Situ hybridization -- _tRecombinant DNA and genetic engineering -- _tFundamentals of recombinant DNA technology -- _tEnzymes in molecular cloning -- _tGene constructs and cloning vectors -- _tDNA libraries -- _tMolecular biology of gene transfer systems -- _tSelection and screening of recombinant molecules -- _tApplications of biotechnology -- _tGenetic engineering of microorganisms -- _tGenetic engineering of animals -- _tGenetic engineering in plants -- _tWorking with proteins -- _tProtein purification techniques -- _tProtein detection and estimation -- _tProtein fractionation techniques -- _tImmunochemical techniques -- _tBacterial and mammalian cell culture -- _tBiology of bacteria -- _tCultivation of mammalian cells in vitro -- _tIn Vitro plant cell culture and crop improvement -- _tPlant cell culture laboratory and requirements -- _tPlant culture media, preparation, and culture initiation -- _tMicropropagation -- _tCultures of organized tissues -- _tCulture of unorganized tissues -- _tCryopreservation and distribution of clonal material -- _tMeasurement of plant cell growth and cytological analysis --
505	8	0	_tProtoplast fusion and somaclonal variation -- _tApplication of plant cell, tissue and organ culture -- _tEnvironmental biotechnology -- _tBiotechnology in pollution control -- _tBiodiversity and genetic conservation -- _tBioenergy fuel from biomass -- _tRegulatory aspects of using genetically-modified organisms -- _tIntellectual property rights and socio-legal aspects of biotechnology.
650		0	_aBiotechnology. _9209212
830		0	_aBiotechnology (Universities Press) _9209214
942			_2ddc _cBK
999			_c70685 _d70685