000			03480fam a2200397 a 4500
001			12088
003			BD-RjUL
005			20211208235231.0
008			940722s1985 enka 00| 0 eng d
020			_a0521304784
020			_a0521315166
035			_a(OCoLC)30919364
035			_a(OCoLC)ocm30919364
035			_a(BD-RjUL)12137
035			_a(NNC)1593901
040			_aDLC _cDLC _dDLC$dOrLoB _dBD-RjUL _beng
041			_aeng
082	0	0	_a581.0724 _220 _bDOE 1985
100	1		_aDodds, John H. _937444
245	1	0	_aExperiments in plant tissue culture / _cJohn H. Dodds and Lorin W. Roberts.
246	3	0	_aPlant tissue culture
250			_a2nd ed.
260			_aCambridge; _aNew York : _bCambridge University Press, _c1985
300			_axiv, 232 p. : _bill. ; _c24 cm.
504			_aIncludes bibliographical references and indexes.
505	0		_aForeword / J. Heslop-Harrison -- I. Background information. 1. Culture of plant cells, tissues, and organs. 2. Laboratory facilities. 3. Aseptic techniques. 4. Media composition and preparation -- II. Experimental: Callus and callus-derived systems. 5. Initiation and maintenance of callus. 6. Organogenesis. 7. Cell suspensions. 8. Somatic embryogenesis -- III. Experimental: Culture of organs and organized systems. 9. Isolated roots. 10. Micropropagation by bud proliferation. 11. Anther and pollen cultures -- IV. Experimental: Isolated cells. 12. Transdifferentiation of parenchyma cells to tracheary elements. 13. Isolation and culture of protoplasts. 14. Protoplast fusion and somatic hybridization -- V. Supplementary topics. 15. Cryopreservation of germplasm. 16. Production of secondary metabolites. 17. Quantitation of procedures.
520			_aThe third edition of Experiments in Plant Tissue Culture makes available new and updated information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This laboratory text features an enlarged section on terminology and definitions as well as additional information on equipment and facilities.
520	8		_aThis comprehensive text takes the reader through a graded series of experimental protocols and also provides a preliminary review of each topic. After an introductory historical background, there are discussions of a plant tissue culture laboratory, aseptic techniques, and nutritional components of media.
520	8		_aSubsequent chapters are devoted to callus induction, organ formation, cell suspensions, somatic embryogenesis, root cultures, micropropagation, anther and pollen cultures, xylem cell differentiation, isolation and fusion of protoplasts, storage of genetic resources, secondary metabolite production, and quantitation of procedures. A glossary of terms, a list of commercial sources of supplies, and a table of media formulations are included.
520	8		_a. This book offers all the basic experimental methods for the major research areas of plant tissue culture, and will be invaluable to undergraduates and research investigators in the plant sciences. It will also be useful to researchers in related areas such as forestry, agronomy, and horticulture, and to those in commercial houses and pharmaceutical companies interested in acquiring the laboratory techniques.
650	0	0	_aPlant tissue culture _xExperiments. _937445
700	1		_aRoberts, Lorin W. _937446
942			_2ddc _cBK
999			_c12088 _d12088