Molecular biotechnology : principles and practices /
Channarayappa.
- Hyderguda, Hyderabad : Universities Press, c2006.
- 1217 p. : ill. ; 24 cm.
- Biotechnology (Universities Press) .
- Biotechnology (Universities Press) .
Includes bibliographical references and index.
Introduction to biotechnology -- Biotechnology : scope and importance -- Biosafety and good laboratory practices -- Advanced techniques in molecular biology -- Techniques of cell fractionation and centrifugation -- Chemical synthesis of nucleic acids -- DNA chip technology and its potential applications -- Bioinformatics in biotechnology -- Working with nucleic acids -- Isolation of nucleic acids -- Measuring nucleic acid concentration and purity -- Electrophoretic techniques -- DNA sequencing -- Genetic maps and marker analysis -- Polymerase chain reaction (PCR) -- In Situ hybridization -- Recombinant DNA and genetic engineering -- Fundamentals of recombinant DNA technology -- Enzymes in molecular cloning -- Gene constructs and cloning vectors -- DNA libraries -- Molecular biology of gene transfer systems -- Selection and screening of recombinant molecules -- Applications of biotechnology -- Genetic engineering of microorganisms -- Genetic engineering of animals -- Genetic engineering in plants -- Working with proteins -- Protein purification techniques -- Protein detection and estimation -- Protein fractionation techniques -- Immunochemical techniques -- Bacterial and mammalian cell culture -- Biology of bacteria -- Cultivation of mammalian cells in vitro -- In Vitro plant cell culture and crop improvement -- Plant cell culture laboratory and requirements -- Plant culture media, preparation, and culture initiation -- Micropropagation -- Cultures of organized tissues -- Culture of unorganized tissues -- Cryopreservation and distribution of clonal material -- Measurement of plant cell growth and cytological analysis -- Protoplast fusion and somaclonal variation -- Application of plant cell, tissue and organ culture -- Environmental biotechnology -- Biotechnology in pollution control -- Biodiversity and genetic conservation -- Bioenergy fuel from biomass -- Regulatory aspects of using genetically-modified organisms -- Intellectual property rights and socio-legal aspects of biotechnology.